Project *
* Project name Deciphering systemically induced plant responses underlying interaction between root-knot nematode and shoot herbivores
Project Contact Details *
* Person name Crispus Mbaluto
* Email crispus.mbaluto@idiv.de
* Organisation name German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
DatasetDetails *
* Dataset_title Above and belowground systemic induced plant responses to root infection by root-knot nematode and leaf feeding by the potato aphid
* Short_abstract This dataset contains measurements of phytohormones and steroidal glycoalkaloids as plant defense responses induced in tomato after root infection by the root-knot nematode (Meloidogyne incognita) at different life cycle stages (i.e., during root invasion, galling, and reproduction) and leaf infestations by potato aphid (Macrosiphum euphorbiae). Further, it contains data on the number of nymphs produced by adult female aphids after feeding on root-knot nematode infected plants, as well as the number of root galls formed by the nematodes throughout its life cycle. For more details see the publication underlying this data. The dataset contains 8 data files (MiMe_LEAF_phytohormones.csv, MiMe_LEAF_gene_expression.csv, MiMe_LEAF_metabolites.csv, MiMe_ROOT_phytohormones.csv, MiMe_ROOT_gene_expression.csv, MiMe_ROOT_metabolites.csv, MiMe_number of nymphs produced.csv, MiMe_number of galls formed.csv), together with 2 metadata files (MiMe_LEAF_ROOT_Metadata_defense response.csv, and MiMe_Metadata_performance.csv) describing the contents of these files. All files use the character encoding: UTF-8.
* Keywords above-below ground interaction, local and systemic responses, phytohormones, plant-mediated interactions, potato aphid (Macrosiphum euphorbiae), root-knot nematodes (Meloidogyne incognita), steroidal glycoalkaloids, systemic responses
Data ContactDetails *
Contact ( 1 ) *
* Name Crispus Mbaluto
* Email crispus.mbaluto@idiv.de
Data manager true
Data creator true
Data provider true
Data owner false
Organisation ( 1 ) *
* Organisation name German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
Research Group ( 1 ) *
* Research_group Molecular Interaction Ecology
Other-specify
Contact ( 2 ) *
* Name Nicole van Dam
* Email nicole.vandam@idiv.de
Data manager false
Data creator false
Data provider false
Data owner true
Organisation ( 1 ) *
* Organisation name German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
Research Group ( 1 ) *
* Research_group Molecular Interaction Ecology
Other-specify
Contact ( 3 ) *
* Name Ainhoa Martinez-Medina
* Email ainhoa.martinez-medina@idiv.de
Data manager
Data creator
Data provider
Data owner true
Organisation ( 1 ) *
* Organisation name German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
Research Group ( 1 ) *
* Research_group Molecular Interaction Ecology
Other-specify
Study
Omics_type_utilized Transcriptomics
Omics_type_utilized 2 Metabolomics
Other-omics_type
Interaction_level Others
Other-interaction_level root-knot nematode - plant - aboveground herbivore interaction
Study description In this study we investigated the impact of root infection by the root-knot nematode Meloidogyne incognita at different stages of its infection cycle, on tomato leaf defense responses triggered by the potato aphid Macrosiphum euphorbiae. In addition, we analysed the reverse impact of aphid leaf feeding on the root responses triggered by the root-knot nematode. We focused specifically on the signalling pathways regulated by the phytohormones jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and indole-3-acetic acid (IAA) as well as steroidal glycoalkaloids as induced defence compounds. We collected leaf material for molecular and chemical analysis after 24 h of above-ground feeding. Our results show that root-knot nematodes affect the defence responses triggered by above-ground feeding aphids. However, aphid feeding did not systematically alter the root-knot nematode-induced defence responses in roots. Moreover, this interaction between root-knot nematode and aphid via tomato plants depends on the nematodes’ infection cycle stage.
Sample Description
Sample source Plant leaves/roots
SampleSpecies Information ( 1 )
Scientific name Solanum lycopersicum L
Variant name Moneymaker
Organism part Leaves and roots
Source sample label
Replicates number 5
Protocols
Sample Collection Protocol
Sampling location Botanical Garden (Greenhouse) of Leipzig University
Sampling location country Germany
Sampling interval Root and leaf samples were collected after 24 hours of Macrosiphum euphorbiae feeding on tomato plants that were infected with root-knot nematode at different infestation stages (invasion, galling or reproduction).
Strain collection The root-knot nematodes (Meloidogyne incognita) were obtained from Rijk Zwaan in The Netherlands and a colony established in the greenhouse. The initial Macrosiphum euphorbiae colony was kindly provided by Dr. Zeger van Herwijnen (Rijk Zwaan Breeding B.V., De Lier, The Netherlands) and then a colony was established in the iDiv-MIE laboratory.
Cultivation condition The root-knot nematodes were reared on Solanum lycopersicum plants (tomato plants) in the greenhouse. The Macrosiphum euphorbiae colony was maintained using the leaf disc method (Rocca and Messelink, 2017) with slight modifications (see our publication for full details). The entire experiment/bioassay was conducted in a greenhouse under the following conditions; Temperature: Day 26 ̊C / Night 24 ̊C, Photoperiod: Day 16 H / Night 8 H, Relative humidity: 30 %. Plants were supplemented with half strength nutrient solution on weekly basis and watered as required.
Sample amount 100
Sample amount measuring unit mg
Sample material processing
Sample storage location iDiv freezers
Sample storage temperature in degree celsius -80
Extraction Protocol
Extraction procedure See the publication for full details and references
Analytical Techniques
Chromatography technique Liquid chromatography was used for phytohormones (LC/MS EVOQ) and metabolites (LC/MS qToF). See this reference for the specific details: Mbaluto et al. (2020) doi:10.1093/aobpla/plaa029
Mass spectrometry technique Liquid chromatography was used for phytohormones (LC/MS EVOQ) and metabolites (LC/MS qToF). See this reference for the specific details: Mbaluto et al. (2020) doi:10.1093/aobpla/plaa029
Nuclear magnetic resonance technique
Molecular technique CFX 384 Real-Time PCR system was used for gene expression analysis. See this reference for the specific details: Mbaluto et al. (2020) doi:10.1093/aobpla/plaa029
Data Processing Pipeline
Data processing software or scripts or tools Microsoft Excel (pre-processing) MS Data Review software (Bruker MS Workstation, version 8.2) R (version 3.6.1)
Data transformation
Metabolite Identification Process
Identification process information See the publication for full details and references
Data processing software See the publication for full details and references
Data Access Policy *
* Data_access_policy Open (CC BY 4.0)
Additional Information
How_to_cite_dataset Mbaluto C., Martinez-Medina A. & van Dam, N.M. (2021). Above and belowground systemic induced plant responses to root infection by root-knot nematode and leaf feeding by the potato aphid [Dataset]. iDiv Data Repository. https://doi.org/10.25829/idiv.1876-13-8394
Publications_based_on_this_dataset Crispus M. Mbaluto, Esraa M. Ahmad, Anne Mädicke, Katharina Grosser, Nicole M. van Dam & Ainhoa Martínez-Medina (accepted 2021). Induced local and systemic defense responses in tomato underlying interactions between the root-knot nematode Meloidogyne incognita and the potato aphid Macrosiphum euphorbiae. Frontiers in Plant Sciences, 12: 632212. https://doi.org/10.3389/fpls.2021.632212
Projects_that_are_related_to_this_dataset Deciphering systemically induced plant responses underlying interaction between root-knot nematode and shoot herbivores
Additional_information This work was funded by Deutsche Forschungsgemeinschaft (DFG) ST18; and a Deutscher Akademischer Austauschdienst (DAAD) PhD grant to Crispus Mbaluto