Project *
* Project name Deciphering plant induced systemic responses underlying interactions between root-knot nematodes and shoot herbivores
Project Contact Details *
* Person name Crispus Mbaluto
* Email crispus.mbaluto@idiv.de
* Organisation name German Center for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
DatasetDetails *
* Dataset_title Induced defense response in tomato roots by root-knot nematode infestation at different life cycle stages and aboveground insect herbivory
* Short_abstract This data set contain measure defense responses induced in tomato roots by nematodes infestation at different life cycle stages and aboveground herbivore. For more details see the publication underlying this data
* Keywords Root-knot nematodes, Aboveground herbivores, Phytohormone, gene expression, Glycoalkaloids, Tomato plants, Spodoptera exigua
Data ContactDetails *
Contact ( 1 ) *
* Name Crispus Mbaluto
* Email crispus.mbaluto@idiv.de
Data manager true
Data creator true
Data provider true
Data owner
Organisation ( 1 ) *
* Organisation name German Center for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
Research Group ( 1 ) *
* Research_group Molecular Interaction Ecology
Other-specify
Contact ( 2 ) *
* Name Nicole M. van Dam
* Email nicole.vandam@idiv.de
Data manager
Data creator
Data provider
Data owner true
Organisation ( 1 ) *
* Organisation name German Center for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig
* Organisation country Germany
Research Group ( 1 ) *
* Research_group Molecular Interaction Ecology
Other-specify
Study
Omics_type_utilized Transcriptomics
Omics_type_utilized 2 Metabolomics
Other-omics_type
Interaction_level Others
Other-interaction_level root feeding nematode - plant - above-ground herbivore interaction
Study description Our study aim was to analyze the impact of aboveground herbivores on root defense responses triggered by root feeding nematodes at different stages of their lifecycle. We investigated changes in phytohormone and α-tomatine levels, as well as the expression of defense signaling markers and glycoalkaloids metabolism (GAME) genes in roots of Solanum lycopersicum (tomato). The tomato plants were infested with root-knot nematode for 5, 15 or 30 days before being challenged with larvae of Spodoptera exigua aboveground. We collected root material after 24 h of aboveground feeding for molecular and biochemical analysis. Our results show that when root-knot nematodes and S. exigua co-occur on tomato plants, S. exigua herbivory might affect JA-regulated root defense responses induced by root-knot nematodes when the nematodes start to reproduce (30 days post infestation).
Sample Description
Sample source Plant roots
SampleSpecies Information ( 1 )
Scientific name Solanum lycoperiscum
Variant name c.v Money maker
Organism part Root
Source sample label
Replicates number 10
Protocols
Sample Collection Protocol
Sampling location University Leipzig Botanical gardens
Sampling location country Germany
Sampling interval Tomato root samples were collected after 24 hours of Spodoptera exigua larvae feeding on tomato plants that infested with root-knot nematodes was at different infestation stages (invasion, galling and reproduction)
Strain collection The root-knot nematode (Meloidogyne incognita) were obtained from Rijkzwaan in Netherlands and a colony of the same established in the greenhouse. The initial Spodoptera exigua colony was purchased from Entocare CV in the Netherlands and then a colony was established in the iDiv-MIE laboratory
Cultivation condition The root-knot nematodes were reared on Solunum lycoperiscum plants (tomato plants) in the greenhouse. The Spodoptera exigua colony was maintained on artificiail diet at idiv climatic chambers. The experiment was conducted in a greenhouse under the following conditions; Temperature; Day 26 ̊C / Night 24 ̊C, Photoperiod: Day 16 H / Night 8 H , Relative humidity: 30 % Plants were supplemented with half strength nutrient solution on weekly basis and watered as required.
Sample amount 100
Sample amount measuring unit mg
Sample material processing
Sample storage location iDiv freezers
Sample storage temperature in degree celsius -80
Analytical Techniques
Chromatography technique LC-MS qToF
Mass spectrometry technique LC-MS/EVOQ
Nuclear magnetic resonance technique
Molecular technique RT-QPCR
Data Processing Pipeline
Data processing software or scripts or tools Microsoft excel (pre-processing)
Data processing software or scripts or tools 2 MS data Review’ software (Bruker MS Workstation, version 8.2)
Data transformation R
Data Access Policy *
* Data_access_policy Open (CC BY 4.0)
Additional Information
How_to_cite_dataset Crispus Mbaluto., Ainhoa Martínez-Medina., and Nicole M. van Dam (2020) Induced defense response in tomato roots infected by root-knot nematode infection at different life cycle stages and with leaf damage by caterpillar [Dataset]. iDiv Data Repository. https://doi.org/10.25829/idiv.1816-16-2864
Publications_based_on_this_dataset Crispus Mbaluto, Esraa M. Ahmad, Melody Fu, Ainhoa Martinez-Medina & Nicole M. van Dam (in press, 2020) The impact of Spodoptera exigua herbivory on Meloidogyne incognita induced root responses depends on the nematodes’ life cycle stages. AoBPlants.
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